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Titlebook: Eukaryotic Cell Cultures; Basics and Applicati Ronald T. Acton,J. Daniel Lynn Book 1984 The Editor(s) (if applicable) and The Author(s), un

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Senescence, Differentiated Function, and Transformation in Cultured Vascular Endothelial Cellse remedies for . pathologies. Although it may be premature to think that this goal soon may be achieved, investigators who are currently studying this cell culture model are probing some basic questions that are of intrinsic interest to cell culturists and cell biologists. With this as an underlying
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Cytogenetic Effects of Cyclamateshy, cytological staining, cell counting, liquid scintillation and karyotyping were used to study the cytogenetic damage and biochemical effects of calcium cyclamate when assayed in 24 hour intervals for 96 hours. The cells were exposed to 10. and 10. molar concentrations of calcium cyclamate in TC 1
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A High Efficiency Stirrer for Suspension Cell Culture with or without Microcarriersary motion arises from the viscous drag from the wall and bottom of the flask. The culture medium is rotated by a bulb-ended rod suspended from inside the flask cap: the bulb orbits around in a circular trough farmed between a central indent in the base and the rounded periphery of the base. There a
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Alternative Surfaces for Microcarrier Culture of Animal CellsThe microcarriers were compared as substrates for the growth of several types of cells and with respect to binding of proteins from the culture medium. Crosslinked dextran has been found to be the most suitable material for a microcarrier matrix and was used as the matrix for the new microcarriers.
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In Vitro and in Vivo Effects of Endothelial Cell-Derived Growth Factorl-derived growth factor (ECDGF), can replace the requirement of 3T3 and smooth muscle cells for exogenous mitogens. ECDGF, added to plasma-derived serum, a platelet deficient preparation of plasma, supports growth of these cells to confluency. Lyophilized preparations of the ECDGF stimulate migratio
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Production of Gamma (Immune) Interferon by a Permanent Human T-Lymphocyte Cell Line or splenic mononuclear cells. A human “helper-inducer” T-lymphocyte cell line (Mo) is an inducible source of gamma interferon. Mo gamma interferon has been biochem ically characterized and partially purified, and resembles the gamma interferon species derived from normal cell sources.
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