Titlebook: Erythropoiesis; Methods and Protocol Joyce A. Lloyd Book 2018 Springer Science+Business Media LLC 2018 Animal models.Red blood cells.Progen

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Chung-Hua Shen,Qi Liang,Xiang-Chao Haot. During the past decade, considerable progress has been made on the development of flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) methods for the analysis and isolation of both murine and human erythroid cells at distinct stages of erythropoiesis, based on changes in the expre

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reticulocyte. Herein, we describe the flow cytometry-based assays for enucleation assessment. The separation of nucleated erythroblasts, reticulocytes, and extruded nuclei by flow cytometry is based on DNA staining, surface expression of erythrocyte specific markers, or forward scatter (FSC). The en

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Jerry McBeath,Jenifer Huang McBeathNA and protein with bi-functional reagents such as formaldehyde and precipitation of the protein with a specific antibody permit PCR amplification (ChIP) or sequencing (ChIP-seq) to identify the bound sites. Here, we present methodology for these approaches that are widely applicable to erythroid ce