| 书目名称 | Erythropoiesis | | 副标题 | Methods and Protocol | | 编辑 | Joyce A. Lloyd | | 视频video | | | 概述 | Includes cutting-edge methods and protocols for the study of erythropoiesis.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expert | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Erythropoie | | 出版日期 | Book 2018 | | 关键词 | Animal models; Red blood cells; Progenitor cell status; Erythroid cells; Chromatin immunoprecipitation ( | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-7428-3 | | isbn_softcover | 978-1-4939-8483-1 | | isbn_ebook | 978-1-4939-7428-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media LLC 2018 |
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发表于 2025-3-21 17:21:05
