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Titlebook: Epstein-Barr Virus Protocols; Joanna B. Wilson,Gerhard H. W. May Book 2001 Humana Press 2001

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Book 2001tion of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor’s etiology (Nature194:232–234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA tech
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Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids the EBV nuclear antigens EBNA2, EBNA3a, and EBNA3c, and the latent membrane protein 1 (LMP1) (.–.). Since EBNA1 maintains the status of the EBV genomes in the proliferating B cells, it might also be indispensable (.).
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Visualizing EBV Expression Patterns by FISH has also been applied to visualization of specific viral RNAs within the nuclei of cells latently infected with EBV (.,.). . two-color detection of EBV-specific nuclear RNA allows the study of different viral expression programs at the cellular level (.).
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https://doi.org/10.1007/978-1-4757-9191-4 assay include an increased sensitivity and a decreased time required to analyze samples relative to DNA blots, the traditional assay used to study replication of oriP-containing plasmids in the presence of EBNA-1 (.–.).
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Annegamento Soccorso tecnico e sanitariousions as to whether this is due to the presence of the virus in the tumor cell population or to the presence of EBV-carrying “by-stander” B cells in the tissue. For a meaningful analysis of EBV infection, it is therefore necessary in many instances to establish the cellular location of the virus using morphology-based techniques.
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