Titlebook: Epstein-Barr Virus Protocols; Joanna B. Wilson,Gerhard H. W. May Book 2001 Humana Press 2001

Coma704

Detection of Epstein-Barr Virus DNA and Viral Gene Productsers of EBV-carrying B cells have been identified in the peripheral blood as well as in lymphoid and nonlymphoid tissues of chronic virus carriers (.,.). This is relevant to the study of human tumors. The detection of EBV DNA in a tumor by polymerase chain reaction (PCR) usually does not permit concl

Generic-Drug

Anonymous

spinal-stenosis

止痛药

Virus Isolationer spontaneously move from latency into a lytic cycle of replication in a very small percentage of the population of most B-cell lines in culture and this number can, with varying degrees of efficiency, be increased by use of inducing agents. Typically, current production methods involve induction o

ALIBI

入伍仪式

伪造者

Introduction of Plasmid Vectors into Cells Via Electroporation viral protein on the host cell separately from the effects of other viral proteins that are being concurrently expressed during a normal infection. Electroporation is a method of introducing a plasmid expressing the viral protein of interest into cells and, for long-term studies, generating cell li

oracle

Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Geneinoma). Among about 90 genes encoded by the virus (.), seven latent genes—(Epstein-Barr viral nuclear antigen 1 (EBNA1), EBNA2, EBNA3A, EBNA3C, latent membrane protein 1 (LMP1), and EBERs—were found to be indispensable for B cell immortalization (.). LMP1 induces malignant transformation in rodent c

Priapism

Transient Gene Expression and MACS Enrichment the efficiency of the transfection method. Lymphoid cells are effectively transfected by electroporation (.–.). However, the transfection efficiency varies usually between 5 and 20% of the living cells, depending on the cell line used. In the case of primary lymphoid cells, the transfection efficie