Titlebook: Epitope Mapping Protocols; Glenn E. Morris Book 1996 Humana Press 1996

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Introduction to Time-Delay Systems used to determine the sequence of the fragments recognized by the MAb 4A. Therefore, reaction times and reagents presented in this chapter may require some modification when a different protein antigen is under investigation.

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Proteolytic Fragmentation for Epitope Mapping, used to determine the sequence of the fragments recognized by the MAb 4A. Therefore, reaction times and reagents presented in this chapter may require some modification when a different protein antigen is under investigation.

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Book 1996o­ gists from a wide range of disciplines in which antibodies are used as molecu­ lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoimmune disea

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,Numerical Simulations and Experiments, the chosen reagent, removal of byproducts after the reaction, and immunoassay of the modified protein for the expression of the epitope. The chemical modification is usually performed in solution, but in a convenient variation described here, the antigen is modified when it is already adsorbed to an ELISA plate (.).