Titlebook: Epitope Mapping Protocols; Glenn E. Morris Book 1996 Humana Press 1996

Oration

Introduction to Thin Film Transistorsof peptide release from the free antigen and from the antigen-MAb complex. By analogy to DNase footprinting, a method to localize protein-binding sites on DNA by nuclease digestion of protein-bound DNA (.), proteolysis of antigen-MAb complexes to identify epitopes on protein antigens has been referred to as “protein footprinting” (.).

佛刊

https://doi.org/10.1007/978-981-16-6092-4cyanogen bromide (CNBr) cleaves C-terminal to methionine residues (.), nitrothiocyanobenzoic acid (NTCB) at Cys (.), iodosobenzoic acid (IBA) at Trp (.), and formic acid between Asp-Pro bonds (.). The fragments on Western blots can often be recognized unequivocally using ..s predicted from the sequence.

改革运动

Engulf

COW

Identifying Residues in Antigenic Determinants by Chemical Modification, the chosen reagent, removal of byproducts after the reaction, and immunoassay of the modified protein for the expression of the epitope. The chemical modification is usually performed in solution, but in a convenient variation described here, the antigen is modified when it is already adsorbed to an ELISA plate (.).

Obvious

一夫一妻制

Epitope Mapping by Chemical Fragmentation,cyanogen bromide (CNBr) cleaves C-terminal to methionine residues (.), nitrothiocyanobenzoic acid (NTCB) at Cys (.), iodosobenzoic acid (IBA) at Trp (.), and formic acid between Asp-Pro bonds (.). The fragments on Western blots can often be recognized unequivocally using ..s predicted from the sequence.

窝转脊椎动物

Epitope Mapping Using Phage-Displayed Peptide Libraries,tein folding. These determinants are present on native protein, but are lost on denaturation or fragmentation. Linear or continuous epitopes contain at least four to six adjacent amino acid residues of the primary sequence and can be identified on denatured as well as native protein (.–.).

MIRE

1064-3745 ed by biolo­ gists from a wide range of disciplines in which antibodies are used as molecu­ lar reagents. The epitope mapper may be interested in studying protein-protein interactions, in developing an immunoassay, in producing protective peptide vaccines, in investigating the pathogenesis of autoim

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