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Titlebook: Epithelial Cell Culture Protocols; Scott H. Randell,M. Leslie Fulcher Book 2013Latest edition Springer Science+Business Media, LLC 2013 ce

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书目名称Epithelial Cell Culture Protocols
编辑Scott H. Randell,M. Leslie Fulcher
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epithelial Cell Culture Protocols;  Scott H. Randell,M. Leslie Fulcher Book 2013Latest edition Springer Science+Business Media, LLC 2013 ce
描述.Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. .Epithelial Cell Culture Protocols, Second Edition. provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting
出版日期Book 2013Latest edition
关键词cell systems; epithelial cells; epithelial interfaces; epithelial monolayers; keratinocyte culture; Cell
版次2
doihttps://doi.org/10.1007/978-1-62703-125-7
isbn_softcover978-1-4939-5679-1
isbn_ebook978-1-62703-125-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2013
The information of publication is updating

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The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eyring these cells from recently deceased adult human donors is the ideal source for disease studies. Adult RPE could also be used as a cell source for transplantation therapy for RPE degenerative disease, likely requiring first in vitro expansion of the cells obtained from a patient. Previous protoco
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Oral and Pharyngeal Epithelial Keratinocyte Culture,ly utilized in multiple research efforts, ranging from the study of oral biology, mucosal immunity, and carcinogenesis to stem cell biology and tissue engineering. This chapter describes step-by-step protocols for the successful isolation and culture of human oral epithelial cells and fibroblasts, a
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Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs, lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.
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