Titlebook: Epithelia; Advances in Cell Phy Christopher J. Jones Book 1990 Springer Science+Business Media Dordrecht 1990 cell.cell culture.cell physio

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Transepithelial Ion Transport in Cultured Colonic Epithelial Cell Monolayersh cultured epithelia to consider both the advantages and disadvantages of such a step and particularly if the questions to be posed are most appropriately answered by such an approach. There are both advantages and disadvantages in using culture systems.

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Electrophysiology of the Nephron: New Insights gained from the Patch-clamp Technique. As far as the transport of ions is concerned, it is thought that the majority of the transepithelial flux passes through (transcellular) rather than between (paracellular) the tubule cells. It is obvious, however, for those ions which undergo transcellular transport, that they must traverse both the apical and basolateral membranes.

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Michael Falk,Frank Marohn,Rainer Beckeron are many and for the purposes of the present chapter emphasis will be placed on the normal and, by way of example, the epithelial abnormality in cystic fibrosis, a condition in which there has been much recent progress.

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Transport Physiology of Renal and Intestinal Cell Culture Modelsecond system is the use of ., in which cells are maintained in culture for extended periods. This chapter will focus on the utility of the latter cell type, as a large number of established cell lines are available (see Table 7.1), and this type of system offers the most experimental flexibility.