Titlebook: Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases; Barbara Stefanska,David J. MacEwan Book 2017 The Editor(s) (i

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High-Throughput Techniques for DNA Methylation Profiling,BS)..An overview of each protocol, including description as to why particular steps are required or critical, is outlined. Further, the protocols are compared and advantages and disadvantages of each are discussed.

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High-Throughput Techniques for DNA Methylation Profiling,er include enrichment-based method, Methylated DNA Immunoprecipitation (MeDIP), paired with microarray technology and next generation sequencing, and sodium bisulfate-based techniques including Infinium HumanMethylation450 BeadChip (Illumina 450 K) and Reduced Representation Bisulfite Sequencing (RR

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Analyzing Targeted Nucleosome Position and Occupancy in Cancer, Obesity, and Diabetes,ithin DNA is important to understanding the potential epigenetic mechanisms of gene regulation during various environmental exposures and diseased states. Targeted nucleosome mapping is a convenient method to map nucleosome positions at a specific genomic locus. In this method, mononucleosomal DNA i

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Synthesis and Application of Cell-Permeable Metabolites for Modulating Chromatin Modifications Regurome-associated mutations in genes encoding key enzymes of intermediary metabolism. Here, we provide an outline for the synthesis of cell-permeable forms of the cellular metabolites (.)-2-hydroxyglutarate and (.)-2-hydroxyglutarate, and their application for the inhibition of α-ketoglutarate-depende

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High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Usinon, it is not commonly used for small molecule screening. More commonly, high throughput screens (HTSs) designed to detect transcriptional changes use a gene reporter, such as green fluorescent protein (GFP), β-galactosidase, or luciferase. The downsides of this approach include the genetic manipula