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Titlebook: Epigenetics Protocols; Trygve O. Tollefsbol Book 2004 Humana Press 2004

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书目名称Epigenetics Protocols
编辑Trygve O. Tollefsbol
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epigenetics Protocols;  Trygve O. Tollefsbol Book 2004 Humana Press 2004
描述The field of epigenetics has grown exponentially in the past decade, and a steady flow of exciting discoveries in this area has served to move it to the forefront of molecular biology. Although epigenetics may previously have been considered a peripheral science, recent advances have shown considerable progress in unraveling the many mysteries of nontraditional genetic processes. Given the fast pace of epigenetic discoveries and the groundbreaking nature of these developments, a thorough treatment of the methods in the area seems timely and appropriate and is the goal of Epigenetics Protocols. The scope of epigenetics is vast, and an exhaustive analysis of all of the techniques employed by investigators would be unrealistic. However, this ™ volume of Methods in Molecular Biology covers three main areas that should be of greatest interest to epigenetics investigators: (1) techniques related to analysis of chromatin remodeling, such as histone acetylation and methylation; (2) methods in newly developed and especially promising areas of epigenetics such as telomere position effects, quantitative epigenetics, and ADP ribosylation; and (3) an updated analysis of techniques involving DNA
出版日期Book 2004
版次1
doihttps://doi.org/10.1385/1592598285
isbn_softcover978-1-61737-501-9
isbn_ebook978-1-59259-828-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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Analysis of Mammalian Telomere Position Effect,ailed instructions are provided for several more general methods related to human telomeres including terminal restriction fragment analysis and purification of telomeres from digested genomic DNA. This chapter summarizes the techniques currently in use that relate to human telomere position effect.
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Profiling DNA Methylation by Bisulfite Genomic Sequencing, a simple protocol that has consistently worked well in our laboratory. Discussions of potential technical problems and corresponding solutions are also included to facilitate the reproducibility of this protocol.
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https://doi.org/10.1007/3-540-30147-Xcan be performed on native chromatin prepared from cells and tissues, in order to analyze histone methylation and acetylation at specific sites in the genome. We also present different PCR-based assays that can be applied to analyze loci of interest in immunoprecipitated chromatin fractions.
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Site-Specific Analysis of Histone Methylation and Acetylation,can be performed on native chromatin prepared from cells and tissues, in order to analyze histone methylation and acetylation at specific sites in the genome. We also present different PCR-based assays that can be applied to analyze loci of interest in immunoprecipitated chromatin fractions.
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