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Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Nature

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https://doi.org/10.1007/978-3-662-39573-8mistry in archival formalin-fixed and paraffin-embedded skin biopsies from healthy and psoriatic subjects. In addition, we provide a protocol for immunocytochemical detection of S100A7/JAB1 colocalization in S100A7 CRISPR-activated human keratinocyte cell line.
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Genetic Modification of Human Primary Keratinocytes by Lentiviral Vectors,lentiviral vectors, far more efficient as supernatant on keratinocytes. In this chapter we describe improved detailed protocols for stable genetic modification of human primary keratinocytes of the skin or head and neck, in both low- and high-calcium conditions by lentiviral vectors.
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Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures, Due to the covalent biotin modification of proteins, our protocol allows for the complete solubilization of the total cellular protein content in differentiated keratinocytes. Thus, a comprehensive network of potential interactors of a protein of interest can be mapped.
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Book 2020Latest editionuncover the intricacies and regulatory mechanisms underlying epidermal cell development and function. The collection takes a detailed view at techniques currently being applied to this developing field. Written for the highly successful .Methods in Molecular Biology. series, chapters include introdu
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