Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 2014Latest edition Springer Science+Business Media New York 2014 Epidermal cell c

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Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies,o genetically induce clonal labelling of cells and follow their progeny. Here we describe a protocol for temporally controlled clonal labelling and for microdissection of individual mouse hair follicles. We further present staining and visualization techniques used in our lab to analyze clones issued from genetically induced labelling.

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https://doi.org/10.1007/978-1-4939-1224-7Epidermal cell culture; Epidermal stem cells; Human epidermis; In vitro and in vivo studies; Keratinocyt

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Kursad TurksenIncludes cutting-edge methods and protocols on epidermal cell research.Fully updated from the previous editions.Provides step-by-step detail essential for reproducible results.Contains key notes and i

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Allgemeine Physiologie des TodesC) can give rise to specialized cells like keratinocytes and recently emerged as a virtually unlimited source of potential therapeutic cells. However, xenogeneic components in differentiation cocktails have been limiting the clinical potential of hESC-derived keratinocytes (hESCs-Kert). Here, we dem

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Allgemeine Psychosomatische Medizindicine applications. It would be advantageous to develop a methodology to grow adult somatic cells with maximum plasticity in a rapid fashion and in large numbers with minimal manipulation. With routine methods, keratinocytes are cultured in standard amount of medium and passaged with enzymes, and t

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https://doi.org/10.1007/978-3-662-61256-9 typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully basal state followed by subsequent co

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