Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 20051st edition Humana Press 2005 DNA.PAC.PCR.Promoter.gene expression.genes.repr

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https://doi.org/10.1007/978-3-662-41090-5their expression of keratin 19 will be described in detail. The methods include the double labeling of tissues for keratin 19 and label-retaining cells (autoradiography of .H-thymidine) .. The labeling of keratin 19 by immunofluorescence of by flow cytometry is described for cells in vitro.

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Isolation, Characterization, and Culture of Epithelial Stem Cellsoped the method described here to isolate this population of epidermal stem cells (EpiSC). We showed that EpiSC are all keratin 14. and thus of keratinocyte origin and not of mesenchymal or hematopoietic origin. We have also demonstrated that EpiSC can regenerate the epidermis, that they can permane

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1064-3745 ible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.978-1-59259-830-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Allgemeine Eigenschaften der Protoplasten,ormation of the protective barrier is the endpoint of epidermal terminal differentiation and defects in this process are reflected in failure, acceleration, or delay in barrier formation. Hence, these latter assays are of particular value as a rapid initial assay for epidermal developmental defects in genetically modified organisms.

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https://doi.org/10.1007/978-3-642-47418-7The aim of this chapter is to provide the researcher with basic protocols to examine gene expression, cell proliferation, and cell death in the developing zebrafish. Although only a few specific epidermal markers have been identified in the zebrafish thus far, we also provide a list of useful genes for epidermal analysis.