Titlebook: Epidermal Cells; Methods and Protocol Kursad Turksen Book 20051st edition Humana Press 2005 DNA.PAC.PCR.Promoter.gene expression.genes.repr

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Serial Cultivation of Primary Adult Murine Keratinocytesand serially culture adult basal keratinocytes from the epidermis of the mouse in a supplemented culture medium that selects for epithelial lineages and enhances their proliferation while inhibiting differentiation through many passages.

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Analysis of Early Epidermal Development in ZebrafishThe aim of this chapter is to provide the researcher with basic protocols to examine gene expression, cell proliferation, and cell death in the developing zebrafish. Although only a few specific epidermal markers have been identified in the zebrafish thus far, we also provide a list of useful genes for epidermal analysis.

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Brain-Imaging

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In Vivo Labeling and Analysis of Epidermal Stem Cellsulsion and allowed to dry in light tight box for 4 wk at 4°C. After fixation with photographic fixer, the slides were washed, lightly stained, and viewed with a light microscope to observe and quantify cells that retained the labeling. Label-retaining cells were identified by the black silver grains over their nuclei.

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Book 20051st editionpidermal cells and their characterization, and applications in regenerative medicine.These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.