Titlebook: Enzymes, Receptors, and Carriers of Biological Membranes; A Laboratory Manual Angelo Azzi,Urs Brodbeck,Peter Zahler Book 1984 Springer-Verl

先驱

Investigation of Amphiphilic Nature of Different Forms of Acetylcholinesterase from Torpedo Marmoratre attached to collagen-like tails forming a triple helix. DS-AChE is a dimer with a S-value of 6.5S in the presence of Triton X-100 and has a subunit molecular weight of 66,000. By limited digestion of DS-AChE with proteinase K a catalytically active enzyme sedimenting at 7.5S is formed.

Simulate

Separation of Hydrophobic Membrane Proteins by Phase Partition: Characterization by Two-Dimensional e partition. An elegant version of the latter technique was described by Bordier (1981). This consists of using a non-ionic detergent with a cloud-point somewhat higher than room temperature to partition proteins into hydrophobic and hydrophilic fractions.

faction

Gudgeon

自爱

Somber

诱导

MULTI

COMMA

Selective Labeling of the ADP/ATP Translocator with Eosin-5-Maleimidecontains 4 cysteins over 297 amino acids. Masking or modification of sulfydryl groups, which are essential for the nucleotide translocation, causes the inhibition of the translocation activity. At least one sulfydryl group is maked when CAT is bound to the translocator.

使痛苦

Site-Directed Hydrophobic Labeling of Membrane Proteins: NBD-Modification of Bacteriorhodopsinintramembraneous disposition of a protein arises from hydrophobic labeling studies. Those segments of intrinsic membrane proteins that are in close association with the hydrocarbon core of a lipid bilayer may be identified by using hydrophobic photogenerated reagents (Gitler and Bercovici 1980, Brunner and Semenza 1981, Ross et al. 1982).