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Titlebook: Enzyme-linked Immunosorbent Assay (ELISA); From A to Z Samira Hosseini,Patricia Vázquez-Villegas,Sergio O Book 2018 The Author(s) 2018 Side

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书目名称Enzyme-linked Immunosorbent Assay (ELISA)
副标题From A to Z
编辑Samira Hosseini,Patricia Vázquez-Villegas,Sergio O
视频video
概述Includes supplementary material:
丛书名称SpringerBriefs in Applied Sciences and Technology
图书封面Titlebook: Enzyme-linked Immunosorbent Assay (ELISA); From A to Z Samira Hosseini,Patricia Vázquez-Villegas,Sergio O Book 2018 The Author(s) 2018 Side
描述This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologieswith widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELIS
出版日期Book 2018
关键词Side Chain Theory; Antigen-antibody Binding Theory; Radioimmunoassay (RIA); Antibody Production; Humoral
版次1
doihttps://doi.org/10.1007/978-981-10-6766-2
isbn_softcover978-981-10-6765-5
isbn_ebook978-981-10-6766-2Series ISSN 2191-530X Series E-ISSN 2191-5318
issn_series 2191-530X
copyrightThe Author(s) 2018
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发表于 2025-3-22 00:19:38 | 显示全部楼层
General Overviews on Applications of ELISA,nd humoral immunity), diagnosis (pregnancy, cancer and infectious diseases), toxicology, drug monitoring, pharmaceutical industry, and transplantation. Different examples related to each area are explained. ELISA was found to play major roles in all the mentioned disciplines.
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Step by Step with ELISA: Mechanism of Operation, Crucial Elements, Different Protocols, and Insighte washing and blocking agents used in assay procedure. The chapter also reviews widely applied enzymes and substrates with their specific characteristics. To complete the assay, the chapter offers information regarding the stopping procedure and readout techniques such as colorimetric, fluorescence
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2191-530X mmune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book gui
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International Perspectives on Aginggens and antibodies including hydrogen bonding (H-bonding), hydrophobic interaction, ionic attraction, and Van der Waals forces such as London dispersion force, dipole-dipole interaction, and ion-dipole interaction are introduced in great details.
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