Titlebook: Enzyme-Mediated Immunoassay; T. T. Ngo,H. M. Lenhoff Book 1985 Plenum Press, New York 1985 Pet.Plasma.Vine.antibody.biology.cell.cell biol

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https://doi.org/10.1007/978-3-030-43343-7ng the substrate reaction. The result is a system with improved sensitivity and broad flexibility. The a-ELISA serves as a useful means of measuring the isotypic distribution of specific immune responses in many species.

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https://doi.org/10.1057/978-1-137-50956-7ght. Using fluorimetric microscopy, Rotman (1961) was able to detect single molecules of the enzyme β-galactosidase. Under more practical circumstances, the detection limits of fluorimetry are frequently limited by background fluorescence such as that arising from serum or other biological samples.

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Fluorogenic Substrate Labeled Separation-Free Enzyme Mediated Immunoassays for Haptens and MacromoleBy definition, a fluorogenic enzyme substrate should not fluoresce at the wavelength used to monitor the assay; however, its enzymatic product should exhibit strong fluorescence at the appropriate monitoring wavelengths. The substrate-analyte ligand conjugate in FSIA serves as a modified enzyme subs

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