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Titlebook: Enzyme-Mediated Immunoassay; T. T. Ngo,H. M. Lenhoff Book 1985 Plenum Press, New York 1985 Pet.Plasma.Vine.antibody.biology.cell.cell biol

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发表于 2025-3-21 16:16:49 | 显示全部楼层 |阅读模式
书目名称Enzyme-Mediated Immunoassay
编辑T. T. Ngo,H. M. Lenhoff
视频video
图书封面Titlebook: Enzyme-Mediated Immunoassay;  T. T. Ngo,H. M. Lenhoff Book 1985 Plenum Press, New York 1985 Pet.Plasma.Vine.antibody.biology.cell.cell biol
描述T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in
出版日期Book 1985
关键词Pet; Plasma; Vine; antibody; biology; cell; cell biology; development; enzyme; enzymes; radioactivity
版次1
doihttps://doi.org/10.1007/978-1-4684-5012-5
isbn_softcover978-1-4684-5014-9
isbn_ebook978-1-4684-5012-5
copyrightPlenum Press, New York 1985
The information of publication is updating

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https://doi.org/10.1057/9780230355484s in biomedical sciences and has become a standard tool in laboratory medicine. Millions of clinical tests are now routinely performed by using RIA. The rapid and wide acceptance of RIA as a routine clinical method can be attributed to (1) the general applicability of the method, i.e., any compound
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https://doi.org/10.1007/978-1-349-24708-0that binding of an anti-drug antibody to an enzyme labeled with the same drug, modulated (inhibited or activated) catalytic activity. Thus a competitive immunoassay can be developed in which drug in the sample and enzyme-labeled drug compete for antibody. Modulation of enzyme activity is directly re
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https://doi.org/10.1007/978-3-662-62325-1label a ligand analyte (L). Such stable covalent enzyme modulator-ligand conjugates (M-L) is capable of modulating the activity of an indicator enzyme by either causing a significant inhibition of the enzyme activity or a dramatic activation.
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https://doi.org/10.1007/978-3-658-04422-0also known as Substrate-labeled Fluorescent Immunoassay) uses a modified fluorogenic enzyme substrate as a label to form a stable covalent substrate-analyte ligand conjugate. This is in contrast to most enzyme mediated immunoassays which use an enzyme rather than an enzyme substrate as the label (Ng
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Central Asia: The Illusion of a World Orderse vesicles can be prepared as large multilamellar structures containing many internal aqueous compartments or as smaller unilamellar structures with only one internal compartment. If prepared in the presence of marker molecules, such as soluble enzymes, ions or flurophores, the markers are entrappe
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Laboratory based EIA methods have found important applications in the areas of clinical chemistry, veterinary medicine, microbiology, and human fertility. The great versatility of these methods, for both the identification and quantitation of clinically relevant antigens, is illustrated by example
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https://doi.org/10.1057/9780230274044ng with the inherently better speed, stability, and cost have resulted in the commercial production of several EIA systems (Monroe, 1984). Most systems have colorimet-rically monitored the indicating enzyme reaction. With the appropriate considerations, a fluorescence monitor can further enhance the
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