Titlebook: Enzyme Engineering; Methods and Protocol Francesca Magnani,Chiara Marabelli,Francesca Parad Book 2022 The Editor(s) (if applicable) and The

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https://doi.org/10.1007/978-3-319-93946-9gned. On the contrary, the enhanced quality of synthetic DNA libraries makes the exploration of sequence space more efficient. Here, methods for the effective utilization of synthetic DNA libraries are described. The overall procedure allows the generation of ready-to-screen libraries within two weeks from synthetic DNA acquisition.

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Sri Addepalli,Yifan Zhao,Lawrence Tinsleylymer is used during the enzyme immobilization procedure to prevent the subunit dissociation of multimeric enzymes as well as to avoid excessive rigidification of the covalently immobilized enzyme. Finally, the reversible co-immobilization of cofactors has been improved by increasing the reactive groups of the support.

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Expression and In Vivo Loading of De Novo Proteins with Tetrapyrrole Cofactorsns in vivo. In addition, we describe the imaging of hydrophobic proteins and cofactor-rich protein droplets by electron and fluorescence microscopy, and how cofactors can be stripped from the de novo proteins to aid in vitro identification.

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Advanced Enzyme Immobilization Technologies: An Eco-friendly Support, a Polymer-Stabilizing Immobililymer is used during the enzyme immobilization procedure to prevent the subunit dissociation of multimeric enzymes as well as to avoid excessive rigidification of the covalently immobilized enzyme. Finally, the reversible co-immobilization of cofactors has been improved by increasing the reactive groups of the support.

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Francesca Magnani,Chiara Marabelli,Francesca ParadIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/313062.jpg

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https://doi.org/10.1007/978-1-4613-1229-1ties. The large insert size (∼35 kb) of such libraries means they are compatible with downstream expression of large biosynthetic gene clusters (BGCs). This allows the discovery of previously undescribed natural products that would be inaccessible using traditional culture-based discovery pipelines.

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Advances in Swine in Biomedical Researchgent consumption and lower costs than conventional robotic alternatives. Here we describe an example of metagenomic screening for nucleoside 2′-deoxyribosyl transferases using FACS as a more widespread and accessible alternative than microfluidic on-chip sorters. This protocol can be easily adapted