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Titlebook: Enzyme Engineering; Methods and Protocol Francesca Magnani,Chiara Marabelli,Francesca Parad Book 2022 The Editor(s) (if applicable) and The

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发表于 2025-3-21 16:09:07 | 显示全部楼层 |阅读模式
书目名称Enzyme Engineering
副标题Methods and Protocol
编辑Francesca Magnani,Chiara Marabelli,Francesca Parad
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Enzyme Engineering; Methods and Protocol Francesca Magnani,Chiara Marabelli,Francesca Parad Book 2022 The Editor(s) (if applicable) and The
描述This book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobilization of enzymes on sustainable polymers, as well as biocatalytic conversions mediated by homogenous enzymatic preparations or whole cells. Written for the highly successful .Methods in Molecular Biology .series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and timely, .Enzyme Engineering: Methods and Protocols. is an ideal guide for both the novice and the veteran researcher interested in biocatalysis..Chapter 13 is available open access under a CC BY 4.0 license..
出版日期Book 2022
关键词Sustainable industrial processes; Enzyme design; Biochemical reactions; Library preparation and screeni
版次1
doihttps://doi.org/10.1007/978-1-0716-1826-4
isbn_softcover978-1-0716-1828-8
isbn_ebook978-1-0716-1826-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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发表于 2025-3-21 21:58:20 | 显示全部楼层
Resurrecting Enzymes by Ancestral Sequence Reconstructionunderstanding physicochemical properties of proteins, among others. In this context, the aim of this chapter is to present the ASR approach applied to the reconstruction of enzymes—.., proteins with catalytic roles. The spirit of this contribution is to provide a basic, hands-to-work guide for bioch
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Book 2022ly, .Enzyme Engineering: Methods and Protocols. is an ideal guide for both the novice and the veteran researcher interested in biocatalysis..Chapter 13 is available open access under a CC BY 4.0 license..
发表于 2025-3-22 20:31:05 | 显示全部楼层
Entering the Emotional Practices of Teaching the user makes about the likelihood of each recombination event, yet most currently available ASR tools provide limited, if any, opportunities for evaluating indel placement in a reconstructed sequence. Graphical Representation of Ancestral Sequence Predictions (GRASP) is an ASR tool that maps inde
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