Titlebook: Engineering and Analyzing Multicellular Systems; Methods and Protocol Lianhong Sun,Wenying Shou Book 2014 Springer Science+Business Media N

预兆好

Sedative

An Observation Method for Autonomous Signaling-Mediated Synthetic Diversification in ,cation system were diversified into two distinct cell states, high and low, through autonomous signaling-mediated bifurcation, when all cells were initialized to the low state. In this chapter, we illustrate the detailed experimental procedures involved in the initialization of cells and the observation of the phenotypic diversification.

parasite

regale

Freeze

恩惠

creatine-kinase

Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System the interplay between the synthetic gene circuits with underline cellular processes and verify the mechanism determining the pattern formation. Here, we describe simple optical setups for quantitative measurements of the cell density and growth and spatial–temporal dynamic characterization of . pattern formation in soft agar plates.

HUMP

Transcriptome Analysis of a Microbial Coculture in which the Cell Populations Are Separated by a Mem changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we describe a method for transcriptome analysis of a microbial coculture in which two different cell populations are separated by a porous membrane.

deface

充气球

The Characterization of Living Bacterial Colonies Using Nanospray Desorption Electrospray Ionizationmicrobial communities is important for investigating metabolic exchange between species without affecting the viability of the colonies. We describe the protocol for bacterial growth, sample preparation, ambient profiling, and data analysis of microbial communities using nano-DESI MS.