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Titlebook: Engineering and Analyzing Multicellular Systems; Methods and Protocol Lianhong Sun,Wenying Shou Book 2014 Springer Science+Business Media N

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发表于 2025-3-21 16:15:59 | 显示全部楼层 |阅读模式
书目名称Engineering and Analyzing Multicellular Systems
副标题Methods and Protocol
编辑Lianhong Sun,Wenying Shou
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Engineering and Analyzing Multicellular Systems; Methods and Protocol Lianhong Sun,Wenying Shou Book 2014 Springer Science+Business Media N
描述.Engineering Multicellular Systems: Methods and Protocols., focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls..Engineering Multicellular Systems: Methods and Protocols. provide a comprehensive laboratory protocol reference for constructing multicellular systems for various applications..
出版日期Book 2014
关键词Microbial ecosystems; cell-cell interactions; engineering genetic; microbial communities; microbial spec
版次1
doihttps://doi.org/10.1007/978-1-4939-0554-6
isbn_softcover978-1-4939-5272-4
isbn_ebook978-1-4939-0554-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
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Lawrence Freedman,Jeffrey Michaelspes of natural communities might have persisted and evolved. Here, we first discuss important considerations when designing a synthetically engineered system. Then, we describe the steps required to create a two-partner cooperative system based on the yeast ..
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https://doi.org/10.1057/9781137483553 changes in cell physiology. For transcriptome analysis, it is essential that the cell populations in the coculture are harvested separately. Here, we describe a method for transcriptome analysis of a microbial coculture in which two different cell populations are separated by a porous membrane.
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https://doi.org/10.1007/978-3-319-17476-1microbial communities is important for investigating metabolic exchange between species without affecting the viability of the colonies. We describe the protocol for bacterial growth, sample preparation, ambient profiling, and data analysis of microbial communities using nano-DESI MS.
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