Titlebook: Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions; Ang Yan Shan Book 2018 Springer Nature Singapore P

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Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions978-981-13-2188-7Series ISSN 2190-5053 Series E-ISSN 2190-5061

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Designing Hybridization Chain Reaction Monomers for Robust Signal Amplification,as early disease diagnosis. Hybridization chain reaction (HCR) is an attractive isothermal, enzyme-free amplification scheme involving the localised hybridization of metastable DNA hairpins into one-dimensional DNA assembly. One challenge in HCR is the difficulty in converting the molecular assembly

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DNA Split Proximity Circuit as a General Platform for Interrogating Biomolecular Events,er is to integrate the different modules into an overall circuit, termed “split proxity circuit”,for detecting biomolecules and biomolecular events while preserving the self-contained and one-pot feature from the prototype introduced in Chap. ., using the reassembly of the split domains as the mecha

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,DNA Split Proximity Circuit for Visualizing Cell Surface Receptor Clustering—A Case Study Using Hum one-pot molecular toolbox, termed the split proximity circuit (SPC), for the autonomous detection and visualization of cell surface receptor clusters. Detection was powered by antibody recognition and a series of autonomous DNA hybridization to achieve localized, enzyme-free signal amplification. T

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Conclusion and Future Outlooks,The circuit assembles dynamically on the target recognition sites in a self-contained manner to execute localized strand displacement. A recurring theme in this thesis was to minimize circuit leakages and a major contribution of this thesis was the development of several key counter-leakage strategi

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2190-5053 ns in the bulk solution.Proposes design concepts for individ.This book provides essential insights into designing a localized DNA circuit to promote the rate of desired hybridization reactions over undesired leak reactions in the bulk solution. The area of dynamic DNA nanotechnology, or DNA circuits

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