Titlebook: Endophytic Microbes: Isolation, Identification, and Bioactive Potentials; A. Sankaranarayanan,Natarajan Amaresan,Mitesh Kuma Book 2023 The

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Mayka Castellano,Heitor Leal Machadocroorganisms was widespread through the use of PCR and DNA sequencing. 16S rRNA sequencing has played a pivotal role in the accurate identification of microorganisms. The 16S rRNA region is universally distributed among microorganisms and is species-specific.

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Heat-Treatment Method for Isolation of , spp. from Plant Tissuesve and in heat-resistant forms. This state occurs due to unfavorable environmental conditions and hinders the vegetative activity of the cell. Spore-forming . can resist excessive heat due to their chemical composition, therefore, to isolate spore-forming . species, heat shock and ethanol treatment could be the efficient ways.

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https://doi.org/10.1007/978-94-010-1721-3he molecular technique for identifying the fungal endophytes and comprises DNA extraction, PCR amplification, DNA sequencing, and phylogenetic analysis. Its power to elucidate intraspecific and interspecific variation among congeneric species proves it extremely valuable regarding the determination

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Endophytic Microbes: Isolation, Identification, and Bioactive Potentials

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Identification of Fungal Endophytes by 28SrDNA Techniqueparation of PCR products (bands) and (4) cloning of PCR products directly into plasmids (e.g., pMD20-T vector) and transferring into .. . DH5α, and (5) positive clones for different taxa are screened by DNA fingerprinting.

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1949-2448 ation advice from the experts.This volume provides basic insight and protocols relating to endophytic microbes. Chapter are divided into five major sections detailing basic isolation, bioactive metabolites production. endophytism, isolation and identification of endophytes, bioactive potentials, and