Titlebook: Emerging Model Organisms; Wei Wang,Nicolas Rohner,Yongfu Wang Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive l

冒号

切碎

使激动

Brígida Souza,Carlos Eduardo Souza Bezerranes of fish with mutations in genes of interest. Our goal is to provide guidelines for performing functional genetic analyses in . using CRISPR-Cas9 and to highlight the advantages of using this approach.

反复无常

圣歌

中国纪念碑

bacteria

https://doi.org/10.1007/978-3-319-77213-4DNA of dividing killifish cells. After a short in vivo labeling pulse of EdU, we list detailed instructions and important considerations for tissue collection, whole-mount sample preparation, cryo-sectioning, click chemistry development, and confocal imaging in regenerating caudal fins, brain, and spinal cord of the African killifish.

脱落

Melatonin

Whole-Brain Clearing and Immunolabelling in the African Killifish rovide a detailed method to process whole brains for volumetric imaging in the African killifish .. We anticipate this method will facilitate the study of aging and regeneration of adult vertebrate organs.

消毒

Cryopreservation of the Mexican Tetra pace and resources within the facility. To obtain high-quality material, animals need to be in optimal health. This protocol describes the basic care required to ensure healthy fish for sample collection, in addition to the methodology for preservation, quality analysis, and subsequent fertilization.