书目名称 | Emerging Model Organisms | 编辑 | Wei Wang,Nicolas Rohner,Yongfu Wang | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Neuromethods | 图书封面 |  | 描述 | .This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms, setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging, Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the .Neuromethods. series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. ..Authoritative and cutting-edge, .Emerging Model Organisms .aims to be a useful practical guide to researches to help further their study in this field. . | 出版日期 | Book 2023 | 关键词 | Tol2-mediated transgenesis; CRISPR; Nothobranchius furzeri; regenerative medicine; gene knockdown | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-2875-1 | isbn_softcover | 978-1-0716-2877-5 | isbn_ebook | 978-1-0716-2875-1Series ISSN 0893-2336 Series E-ISSN 1940-6045 | issn_series | 0893-2336 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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