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Titlebook: Embryonic Stem Cell Protocols; Volume II: Different Kursad Turksen Book 2006Latest edition Humana Press 2006 Vivo.biology.cell.cell culture

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Neural Stem Sphere Methodosed of three culture stages. In the first stage, floating ES cell colonies are cultured in astrocyte-conditioned medium to directly differentiate neural stem cells from ES cells, resulting in neural stem spheres. In the second stage, neural stem spheres are cultured as adhesive cells to produce neu
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Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In hite matter and divide a limited number of times before they terminally differentiate. Thus far, it has been possible to purify OPCs only from the rat optic nerve, but the purified cells cannot be obtained in large enough numbers for conventional biochemical analyses. Moreover, the central nervous s
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Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer blueprint of an organism. These fascinating cells are bestowed with pluripotent characteristics and can be directed to differentiate into various lineages in vitro. Hence, these cells are being explored as an ideal in vitro model in gaining insight into early developmental events. Using the ES cell
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Optimized Neuronal Differentiation of Murine Embryonic Stem Cellsiety of protocols have been described to facilitate neuronal differentiation. The density of ES cells used for neuronal differentiation has striking effects on the proportion and purity of the derived neuronal cells. Here, the protocols used to optimize ES cell density in neuronal differentiation wi
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Generation of Inner Ear Cell Types From Embryonic Stem Cellshair cells and other cell types of the inner ear results in hearing and balance disorders that substantially diminish the quality of life. The irreversibility of hearing loss in mammals is caused by the inability of the cochlea to replace lost hair cells. No drugs are available that stimulate inner
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The Role of , in Hemangioblast and Hematopoietic Developmentice, however, in vitro embryonic stem cell differentiation assays were employed to elucidate the role of . in murine hematopoiesis. These studies revealed that . is not required for hemangioblast development but is required in a dose-dependent fashion for hemangioblast differentiation to definitive
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