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Titlebook: Embryonic Stem Cell Protocols; Volume II: Different Kursad Turksen Book 2006Latest edition Humana Press 2006 Vivo.biology.cell.cell culture

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书目名称Embryonic Stem Cell Protocols
副标题Volume II: Different
编辑Kursad Turksen
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Embryonic Stem Cell Protocols; Volume II: Different Kursad Turksen Book 2006Latest edition Humana Press 2006 Vivo.biology.cell.cell culture
描述Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
出版日期Book 2006Latest edition
关键词Vivo; biology; cell; cell culture; cell differentiation; development; neural stem cells; protein; stem cell;
版次2
doihttps://doi.org/10.1385/1597450367
isbn_softcover978-1-61737-777-8
isbn_ebook978-1-59745-036-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2006
The information of publication is updating

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https://doi.org/10.1057/9781403982322ffects on the proportion and purity of the derived neuronal cells. Here, the protocols used to optimize ES cell density in neuronal differentiation with and without an initial aggregation step are described.
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https://doi.org/10.1057/9781137364937aled that . is not required for hemangioblast development but is required in a dose-dependent fashion for hemangioblast differentiation to definitive hematopoietic progenitors. Contained here are the methods needed to conduct embryonic stem cell-derived in vitro hematopoietic assays.
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Optimized Neuronal Differentiation of Murine Embryonic Stem Cellsffects on the proportion and purity of the derived neuronal cells. Here, the protocols used to optimize ES cell density in neuronal differentiation with and without an initial aggregation step are described.
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https://doi.org/10.1007/978-981-16-5485-5enic differentiation in EBs by histochemical staining, immunostaining, mRNA-. hybridization, and reverse transcriptase polymerase chain reaction analysis. Here, we describe in detail our established protocols to analyze chondrogenic differentiation of ES cells. We summarize different ways to modulate ES cell-derived chondrogenic differentiation.
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https://doi.org/10.1007/978-3-531-93267-5anced with a high frequency of K14-positive cells. Thus, the in vitro co-culture model system described here is useful in dissecting the process of commitment and differentiation of epithelial progenitors that occurs during epidermogenesis as well as for the investigation of the molecular mechanisms underlying these processes.
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