Titlebook: Electroporation and Sonoporation in Developmental Biology; Harukazu Nakamura Book 2009 Springer-Verlag Tokyo 2009 Expression.RNA.Tempo.Ver

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The Future of Earth’s Climate After Parisiwa et al., 1991; Sato et al., 2007). We applied this system for tracing retinal fibers (Harada et al., 2008). In this chapter, we demonstrate the method of tracing retinal fibers from both small and large groups of the retinal ganglion cell (RGC) with transposon-mediated gene transfer by in ovo electroporation to chick embryos.

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Book 2009 and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of

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Book 2009the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced. .

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Electroporation and Sonoporation in Developmental Biology

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Electroporation and Sonoporation in Developmental Biology978-4-431-09427-2

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