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Titlebook: Electroporation and Sonoporation in Developmental Biology; Harukazu Nakamura Book 2009 Springer-Verlag Tokyo 2009 Expression.RNA.Tempo.Ver

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In Utero Electroporation: Assay System for Migration of Cerebral Cortical Neuronse and then migrate toward the pial surface to form the highly organized 6-layered cerebral cortex. Detailed profiles of these processes and their molecular mechanisms had been largely unknown because of the absence of an efficient assay system. The in vivo electroporation system was initially devise
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Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Culturesrevealing many neural connection patterns in the CNS (Itaya and van Hoesen, 1982; Fabian and Coulter, 1985; Yoshihara, 2002). Moreover, a tracing method with fluorescent dye has enabled the observation of growing axons in living conditions, and demon strated a lot of developmental aspects in axon gr
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Gene Transfer into Mouse Haemogenic Sites, as a Mean to Functionally Approach the Control of Mesoderf two independently generated pools of haematopoietic precursors. These two haematopoietic precursors waves are dedicated to specialized functions regarding haematopoiesis ontogeny. The first, which occurs in the extra-embryonic compartment, in the yolk Sac (YS) blood islands, rapidly produces the d
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The External Accounting Framework,of in ovo electroporation. Here, we show the application of in ovo electroporation to the developmental neural tube in chick embryo. electroporation, only the anode side is transfected and the cathode side could be used as the control (Funahashi et al., 1999; Nakamura et al., 2000; Odani et al., 2008).
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