Titlebook: Electroporation Protocols for Microorganisms; Jac A. Nickoloff Book 1995 Humana Press 1995

假

缩减了

过份艳丽

Justification for Early Surgery,hat can be applied to eggs, sperm, platelets, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, and yeast—generally to any vesicle surrounded by a membrane. The term “cells” will be used representatively for any of the vesicles to be manipulated unless specific requirements dictate otherwise.

一骂死割除

整体

慷慨不好

AVOID

Instrumentation,hat can be applied to eggs, sperm, platelets, mammalian cells, plant protoplasts, plant pollen, liposomes, bacteria, fungi, and yeast—generally to any vesicle surrounded by a membrane. The term “cells” will be used representatively for any of the vesicles to be manipulated unless specific requirements dictate otherwise.

Default

Electrotransformation in ,,rom 10. to 10. transformants/µg of covalently closed circular plasmid DNA, but efficiency is frequently lower because these methods are very sensitive to purity of reagents, cleanliness and quality of glassware and plasticware, and methods of cell handling (.).

opportune

危险

Electrotransformation of ,,uce exogenous DNA into these organisms is important in establishing genetic linkages to virulence and pathogenicity factors in some species, and the ability to stabilize or improve other phenotypic traits in other species.