Titlebook: Electron Microscopy Methods and Protocols; M. A. Nasser Hajibagheri Book 19991st edition Humana Press 1999

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General Preparation of Material and Staining of Sections,rent types of mammalian material for ultrastructural examination; for processing of plant material (. Hall and Hawes, ..). The material to be processed may be cell suspensions, particulates, monolayer cultures, or tissue derived from organs. The former three must initially be processed differently f

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Negative Staining of Thinly Spread Biological Particulates,enner and Horne in 1959 (.). Rapidly, this staining technique was applied to other biological particulates, usually when a purified or semipurified aqueous suspension of freely suspended (i.e., nonaggregated) material was available. In addition to viruses, this material ranged from purified enzymes

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The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use inhe antibodies bind specifically to the protein being investigated and electron opaque markers visualize their location within the cell. For electron microscopy, it is important to have preparation methods that open the cells and allow access of antibodies to the proteins (or antigens) they bind to w

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High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immuicant ice crystal damage. Other freezing methods are limited to 1/100 (plunge and impact freezing) to 1/10 (propane jet freezing) that sample thickness. With HPF you are working in the realm of whole cells and tissues instead of small cells or cell cortical regions. For more details on the theory of