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Titlebook: Electron Microscopy Methods and Protocols; M. A. Nasser Hajibagheri Book 19991st edition Humana Press 1999

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Electron Microscopic Enzyme Cytochemistry,enzyme assay in test tubes. Immunocytochemistry detects the enzyme molecule itself, but gives no information about enzyme activity. Instead, immunocytochemistry is more correlative to immnological techniques such as Western blotting. Thus, enzyme cytochemistry shows distinct biological significance from immunocytochemistry.
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The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use inicroscopy, it is important to have preparation methods that open the cells and allow access of antibodies to the proteins (or antigens) they bind to without destroying the normal cellular organization (or morphology). If possible, the data obtained from immunocytochemical experiments should be quantitative (. .).
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High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immus. With HPF you are working in the realm of whole cells and tissues instead of small cells or cell cortical regions. For more details on the theory of how HPF works, and some examples of its application, there are several reviews available (.-.).
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,Professor Meghnad Saha (1893–1956),ents, and microtubules, membrane systems, liposomes, and many different virus particles. Detailed surveys of the technical, methodological considerations, and applications of cryoelectron microscopy have been presented (.,.).
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