Titlebook: Electron Microscopy; Methods and Protocol John Kuo Book 2014Latest edition Springer Science+Business Media, New York 2014 (EM) data.Cryo-sp

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Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specime are described for handling cells grown in liquid culture as well as on substrates such as culture dishes, slide culture chambers, or agar. These protocols may be used to process not only cultured organisms but also larger botanical and zoological specimens.

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High-Pressure Freezing: Current State and Future Prospects,l due to significant changes compared to its predecessor model. Its centerpiece is a multipart polymer cartridge which holds the specimen carrier sandwich and guides it automatically through the freezing process until immersed in liquid nitrogen. The cartridge can be adapted to the specimen and carr

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Cryo-electron Microscopy of Membrane Proteins,tubular crystals. This method has been used to determine the atomic resolution structures of bacteriorhodopsin, tubulin, aquaporins, and several other membrane proteins. In addition, a large number of membrane protein structures were studied at a slightly lower resolution, whereby at least secondary

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Tracking DNA and RNA Sequences at High Resolution,ed primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most f