书目名称 | Electron Microscopy | 副标题 | Methods and Protocol | 编辑 | John Kuo | 视频video | | 概述 | Deals with biological and molecular research techniques based on TEM and SEM.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the exper | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This third edition of .Electron Microscopy: Methods and Protocols .expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting | 出版日期 | Book 2014Latest edition | 关键词 | (EM) data; Cryo-specimen; DNA and RNA tracking; Focussed Ion Beam (FIB); Scanning Electron Microscope (S | 版次 | 3 | doi | https://doi.org/10.1007/978-1-62703-776-1 | isbn_softcover | 978-1-4939-6073-6 | isbn_ebook | 978-1-62703-776-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, New York 2014 |
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