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Titlebook: Electron Microscopy; Methods and Protocol John Kuo Book 2014Latest edition Springer Science+Business Media, New York 2014 (EM) data.Cryo-sp

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发表于 2025-3-21 16:06:58 | 显示全部楼层 |阅读模式
书目名称Electron Microscopy
副标题Methods and Protocol
编辑John Kuo
视频video
概述Deals with biological and molecular research techniques based on TEM and SEM.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the exper
丛书名称Methods in Molecular Biology
图书封面Titlebook: Electron Microscopy; Methods and Protocol John Kuo Book 2014Latest edition Springer Science+Business Media, New York 2014 (EM) data.Cryo-sp
描述.This third edition of .Electron Microscopy: Methods and Protocols .expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting
出版日期Book 2014Latest edition
关键词(EM) data; Cryo-specimen; DNA and RNA tracking; Focussed Ion Beam (FIB); Scanning Electron Microscope (S
版次3
doihttps://doi.org/10.1007/978-1-62703-776-1
isbn_softcover978-1-4939-6073-6
isbn_ebook978-1-62703-776-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, New York 2014
The information of publication is updating

书目名称Electron Microscopy影响因子(影响力)




书目名称Electron Microscopy影响因子(影响力)学科排名




书目名称Electron Microscopy网络公开度




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书目名称Electron Microscopy年度引用




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书目名称Electron Microscopy读者反馈




书目名称Electron Microscopy读者反馈学科排名




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A ‘Soldier of the Third International’paration of APS-mica surfaces and the preparation of samples for AFM imaging. The protocol for synthesis and purification of APS is also provided. The AFM applications are illustrated with examples of images of DNA and protein–DNA complexes.
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History of Social Law in Germanyhods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.
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Tracking DNA and RNA Sequences at High Resolution,hods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail.
发表于 2025-3-22 17:23:42 | 显示全部楼层
Biological Applications of Phase-Contrast Electron Microscopy,Zernike PC cryo-electron microscopy to biological systems such as protein molecules, virus particles, and cells, including single-particle analysis to delineate three-dimensional (3D) structures of protein and virus particles and cryo-electron tomography to reconstruct 3D images of complex protein systems and cells.
发表于 2025-3-23 00:43:25 | 显示全部楼层
1064-3745 .Contains key notes and implementation advice from the exper.This third edition of .Electron Microscopy: Methods and Protocols .expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related im
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https://doi.org/10.1007/978-3-031-36892-9mples in aluminum specimen carriers, cell cultures grown on Sapphire discs, suspensions for freeze-fracturing, and specimens for cryo-sectioning. Additionally, we include an advanced technique to freeze monolayer cell cultures on Sapphire discs with the Leica EM PACT2 HPF machine using a composite carrier.
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