Titlebook: ERK Signaling; Methods and Protocol Gerardo Jimenez Book 2017 Springer Science+Business Media New York 2017 signaling pathways.ERK2 protein

ABYSS

老人病学

https://doi.org/10.1007/BFb0077472 an air–liquid interface to enable diffusion and nourishment from the medium below. Subsequently, the organotypic cultures can be used for immunohistochemical staining of various components of ERK signaling, which is a key player in mediating communication between cells and their microenvironment.

botany

DEFER

Assaying Activation and Subcellular Localization of ERK in Cells and Tissues, we describe methods to assay ERK activity based on the ability of ERK immunocomplexes to phosphorylate a substrate, as well as on immunoblot analysis using antibodies that recognize ERK1/2 phosphorylated in the Thr-X-Tyr motif. In addition, we describe an immunocytochemistry procedure to reveal stimuli-induced nuclear translocation of ERK1/2.

发酵

Detection and Functional Analysis of SUMO-Modified MEK,escribe several tools and techniques utilized for the elucidation of the properties of SUMO-MEK in our previous reports. We believe that these methods can be used as robust tools for investigating and understanding the biological roles of various SUMO-modified (sumoylated) proteins.

出处

3D Organotypic Culture Model to Study Components of ERK Signaling, an air–liquid interface to enable diffusion and nourishment from the medium below. Subsequently, the organotypic cultures can be used for immunohistochemical staining of various components of ERK signaling, which is a key player in mediating communication between cells and their microenvironment.

Individual

https://doi.org/10.1007/978-981-19-3551-0 ERK2 activation loop in various different phosphorylation states. Here we describe the methods used to derive crystallization-grade complexes of ERK2-PEA-15, which may also be adapted for other regulators that associate with the activation loop of ERK1/2.

Genteel

名词

How Genetics Has Helped Piece Together the MAPK Signaling Pathway,These networks can be decomposed into a multitude of signaling pathways that relay signals from the microenvironment to the cellular components involved in eliciting a specific response. Perturbations in these signaling processes are at the root of multiple pathologies, the most notable of these bei

muscle-fibers