Titlebook: ERK Signaling; Methods and Protocol Gerardo Jimenez Book 2017 Springer Science+Business Media New York 2017 signaling pathways.ERK2 protein

anatomical

acheon

Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA),ties in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase–substrate interactions betw

textile

Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors,lls with subcellular resolution. There are quite a number of already existing sensors and this technology is increasingly used to obtain quantitative dynamic datasets. In this chapter, we describe the analysis of endogenous extracellular signal-regulated kinase (ERK) activity in living cells using t

颂扬国家

无聊的人

Co-culture Activation of MAP Kinase in , S2 Cells,ological detection of phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway components, and assess ligand activity. In this chapter, I describe a cell co-culture method to assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK (ex

pacific

费解

Commemorate

蜈蚣

978-1-4939-8195-3Springer Science+Business Media New York 2017

cornucopia

Gerardo JimenezIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts