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Titlebook: ECAT Assay Procedures A Manual of Laboratory Techniques; European Concerted A Jørgen Jespersen,Rogier M. Bertina,F. Haverkate Book 1992 Spr

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发表于 2025-3-21 17:08:40 | 显示全部楼层 |阅读模式
书目名称ECAT Assay Procedures A Manual of Laboratory Techniques
副标题European Concerted A
编辑Jørgen Jespersen,Rogier M. Bertina,F. Haverkate
视频video
图书封面Titlebook: ECAT Assay Procedures A Manual of Laboratory Techniques; European Concerted A Jørgen Jespersen,Rogier M. Bertina,F. Haverkate Book 1992 Spr
描述This book offers a description of current and recently developed laboratory assays in the field of haemostasis and thrombosis. It is the result of a unique cooperation between experts from more than 60 institutes in 12 European countries, brought together by the ECA T (European Concerted Action on Thrombosis and Disabilities) under the auspices of the Commission of the European Communities in Brussels, Belgium. The ECAT, which was initiated in 1981, designed and performed three prospective clinical studies to establish haemostatic factors as risk indicators of thrombosis. Included were patients with angina pectoris at risk from myocardial infarction, patients undergoing angioplasty at risk from re-stenosis, and patients receiving hip replacement at risk from deep venous thrombosis. Assay procedures were chosen, training courses for technicians held, and essential reagents were supplied from a central source. A quality control assessment scheme served to compare assay results both within and between laboratories. In the angina pectoris study, centres determined most of the assays locally; in the other two studies assays were performed centrally. The need for further quality assessme
出版日期Book 1992
关键词Laboratory; Laboratory Techniques; blood; enzymes; plasma; thrombosis
版次1
doihttps://doi.org/10.1007/978-94-011-2992-3
isbn_softcover978-94-010-5330-3
isbn_ebook978-94-011-2992-3
copyrightSpringer Science+Business Media Dordrecht 1992
The information of publication is updating

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发表于 2025-3-21 22:25:58 | 显示全部楼层
Blood collection and preparation: pre-analytical variation,ent handling and plasma storage. The amount of care given to these aspects is ultimately reflected in the accuracy of the result. As soon as blood is withdrawn from a vessel, changes take place in the components of blood coagulation and haemostasis, e.g. platelet activation, release of tissue factor
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Fibrinogen,h half consists of three different polypeptide chains, Aα, Bß and γ, linked together by disulphide bridges. The two sets of chains are connected at the aminoterminal end region by three disulphide bridges, two between the γ-chains and one between the α-chains. This native fibrinogen of 340 kD molecu
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Factor VII clotting activity,5nmol/L). The complex of the zymogen factor VII with tissue thromboplastin and Ca. has been proposed as an efficient activator of factor X. Factor VII can be converted to the two-chain α-VIIa by hydrolysis of an Arg-Ile bond by factors Xa, IXa, XIIa or thrombin. The coagulant activity of α-VIIa is 1
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Factor VIII clotting activity,e ., based oil the thromboplastin generation test. The relative merits of the two assays have been debated. In general, results obtained on the same samples are similar; however, discrepancies can be seen in plasmas which contain activated factors of the coagulation cascade. These factors make the c
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Von Willebrand factor, in the presence of the antibiotic ristocetin; hence, this method is known as ristocetin cofactor activity assay.. The measurement of ristocetin cofactor activity is complicated by the need to prepare a standardized suspension of formaldehyde-fixed normal platelets, which makes the assay unsuitable
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Protein C activity and antigen,vated protein C, APC), mostly in the form of a two-chain molecule consisting of a light chain and heavy chain linked by one disulphide bond. Protein C is synthesized in the liver. During post-translational modification nine glutamic acid residues in the aminoterminus of the light chain are carboxyla
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