Titlebook: Drug Target miRNA; Methods and Protocol Marco F. Schmidt Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 drug

参考书目

Non-nucleotide Modification of Anti-miRNA Oligonucleotides This protocol describes use of ZEN AMOs in a dual-luciferase reporter assay as a simplified means to validate AMO performance or to quickly test putative miRNA binding sites in target sequences. This protocol also describes a method using Western blot analysis for quantifying the level of upregulat

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Rapid Generation of miRNA Inhibitor Leads by Bioinformatics and Efficient High-Throughput Screening ting adaptation of breast cancer cells to hypoxic stress. Herein, we describe a protocol that utilizes bioinformatics to first identify lead small molecules that bind to DICER cleavage sites in pre-miRNAs and then employ an efficient, high-throughput fluorescent-based screening system to determine t

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Mundane

Confidence: The “Flywheel” Turns This protocol describes use of ZEN AMOs in a dual-luciferase reporter assay as a simplified means to validate AMO performance or to quickly test putative miRNA binding sites in target sequences. This protocol also describes a method using Western blot analysis for quantifying the level of upregulat

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,Ausgewählte Konstruktionstheorieen,ting adaptation of breast cancer cells to hypoxic stress. Herein, we describe a protocol that utilizes bioinformatics to first identify lead small molecules that bind to DICER cleavage sites in pre-miRNAs and then employ an efficient, high-throughput fluorescent-based screening system to determine t

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Functional Analysis of miRNAs Using the DIANA Tools Online Suiteal conditions but also as diagnostic biomarkers or promising therapeutic targets. The increased complexity of the miRNA interactomes hinders straightforward interpretation of miRNA expression differences between states and conditions. To this end, functional analysis web servers process and combine

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Non-nucleotide Modification of Anti-miRNA Oligonucleotidescking antisense oligonucleotides (ASOs) that inhibit miRNA function through high-affinity binding and subsequent inactivation and/or degradation of the targeted miRNA. AMOs are a primary tool used to empirically determine the biological targets of a miRNA and can also be used therapeutically when ov

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