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Titlebook: Drosophila Oogenesis; Methods and Protocol Michelle S. Giedt,Tina L. Tootle Book 2023 The Editor(s) (if applicable) and The Author(s), unde

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Exploiting the United Kingdom’s Oil Reserves Studies of live border cell cluster migration have revealed similarities with other migrating collectives, including formation and restriction of cellular protrusions to the front of the cluster, supracellular actomyosin contractility of the entire collective, and intra-collective cell motility. He
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Housing,ermline and somatic cells, the ease of genetic manipulation in fruit flies, and the large number of egg chambers produced by each fly. Recent improvements in live imaging and ex vivo culturing protocols have enabled researchers to conduct more detailed, longer-term studies of egg chamber development
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The Prospects for Global Energy, 1985–2000tion of multiple cellular and molecular events including the spatiotemporal breakdown of the follicle wall. Recent work has shown that ovulation in . utilizes conserved cellular processes and molecular pathways as in mammals. Thus, . ovulation can serve as a good model to decipher the fundamental me
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The Prospects for Global Energy, 1985–2000tem is the ability to capture these dynamic processes in ex vivo organ culture using high-resolution live imaging. Ex vivo culture also allows acute pharmacological or labeling treatments, extending the versatility of the system. However, many current ex vivo egg chamber culture setups do not permit
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The Prospects for Global Energy, 1985–2000s, it is critical for researchers to be able to identify which of their stocks maintain this infection to avoid any potential confounding variables. Here, we describe methods for detecting the bacterium and assessing the infection, including polymerase chain reaction (PCR) of DNA, multi-locus sequen
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The Prospects for Global Energy, 1985–2000ession of transposable elements (TEs) involves the complex PIWI pathway and several proteins associated with heterochromatinization. All players of TE repression are indispensable for proper reproductive fitness, as loss-of-function mutations in these genes result primarily in sterility and impaired
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,,ar heterogeneity makes the ovary a rich subject of study, but also makes it challenging to identify transcriptional differences between individual cell types using methods such as bulk RNA sequencing. The development of single-cell RNA sequencing (scRNA-seq) techniques addresses this limitation by p
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,,ogenesis. In order to understand the molecular basis of chromatin-based processes, such as transcription, it is essential to be able to study how and when different proteins, such as transcription factors, histones and RNA polymerases, interact with chromatin. One of the most popular methods to stud
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