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Titlebook: Drosophila Oogenesis; Methods and Protocol Michelle S. Giedt,Tina L. Tootle Book 2023 The Editor(s) (if applicable) and The Author(s), unde

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书目名称Drosophila Oogenesis
副标题Methods and Protocol
编辑Michelle S. Giedt,Tina L. Tootle
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Drosophila Oogenesis; Methods and Protocol Michelle S. Giedt,Tina L. Tootle Book 2023 The Editor(s) (if applicable) and The Author(s), unde
描述This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Drosophila Oogenesis: Methods and Protocols. serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism..
出版日期Book 2023
关键词Drosophila melanogaster; Imaging techniques; Model organism; Fly oogenesis; CRISPR-mediated mutagenesis
版次1
doihttps://doi.org/10.1007/978-1-0716-2970-3
isbn_softcover978-1-0716-2972-7
isbn_ebook978-1-0716-2970-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Analysis of Physiological Control of Adult , Oogenesis by Interorgan Communication, sensitive to environmental challenges and has recently been shown to be regulated by signaling from peripheral organs. To dissect the intricate coordination between overall organism health and reproduction, it is necessary to meticulously characterize both experimental tools and oogenesis processes
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Immunohistochemical Analysis of Nuclear Lamina Structures in the , Ovary Using CRISPR-Tagged Genes,ver, as females age, fecundity declines, in part, due to changes in ovarian niche function and germline stem cell (GSC) homeostasis. Understanding the dynamics of GSC maintenance will provide needed insights into how coordinated tissue homeostasis is lost during aging. Critical regulators of GSC mai
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Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Millective migration. The basal surface of each follicle cell has lamellipodial and filopodial protrusions that extend from its leading edge and an array of stress fibers that mediate its adhesion to the extracellular matrix; these migratory structures are all globally aligned in the direction of tiss
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