Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2008 Humana Press 2008 Homologous recombination.Microarray.Model organisms.cell lin

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Microscopic Analysis of the Adult , Retina Using Semithin Plastic Sections,rved as an invaluable experimental system to study cell-cell interactions, inductive signaling events, cell proliferation, programmed cell death, cell differentiation, cell organization, and planar cell polarity among others. Importantly, the eye is dispensable for viability and fertility of the fly

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Fluorescent , Hybridization Protocols in , Embryos and Tissues,ethods suffered from limitations in resolution or sensitivity, the recent development of peroxidase-mediated tyramide signal amplification provides strikingly enhanced sensitivity and subcellular resolution. In this chapter, we describe optimized fluorescent . hybridization protocols for . embryos a

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Linear RNA Amplification for the Production of Microarray Hybridization Probes,perimental approach to investigate these processes is to catalog the transcriptome by hybridization of mRNA to DNA microbar-rays. In these experiments mRNA-derived hybridization probes are produced and hybridized to an array of DNA spots on a solid support. The labeled cDNAs of the complex hybridiza

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Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis,e being added to the arsenal of fly geneticists, most recently, the φC31 integrase. This chapter will introduce these recombinases and describe how such instruments are utilized to accurately manipulate the . genome.

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Microscopic Analysis of the Adult , Retina Using Semithin Plastic Sections, and thus, it can easily be manipulated, making it an ideal target for genetic screens. This chapter described an essential technique in the analysis of different genotypes in the adult fly eye, and allows detailed analyses with single cell resolution.

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Fluorescent , Hybridization Protocols in , Embryos and Tissues,nd tissues utilizing tyramide signal amplification, either for single genes or in a high-throughput format, which greatly increases the sensitivity, consistency, economy, and throughput of the procedure. We also describe variations of the method for RNA-RNA and RNA-protein codetection.

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1064-3745 s for live imaging in embryos, larvae, and pupaeDrosophila melanogaster has been the model system of choice for many inv- tigators over the past hundred years. Due to its long-standing tradition as a model organism, many techniques used in Drosophila have been established and c- tinue to be develope

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https://doi.org/10.1007/978-3-642-83771-5his review intends to help . neophytes in setting up a fly lab. It briefly introduces the biological properties of fruit flies, describes the minimal equipment required for working with flies, and offers some basic advice for maintaining fly lines and setting up and analyzing experiments.

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