Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2008 Humana Press 2008 Homologous recombination.Microarray.Model organisms.cell lin

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Linear RNA Amplification for the Production of Microarray Hybridization Probes,ization probes from a few 100 pg of mRNA. These smaller quantities can be isolated from a few 100 cells. Here, we present a linear amplification protocol designed to preserve both the relative abundance of transcripts as well as their sequence complexity.

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https://doi.org/10.1007/978-3-642-84545-1cloning and eventually promoted molecular analysis of a large number of fly genes. Soon after, P-elements were modified to detect genes not only based on a mutant phenotype but rather through revealing RNA or protein expression patterns (enhancer trap, gene trap). Owing to the typically imprecise mo