Titlebook: Downstream Processing of Proteins; Methods and Protocol Mohamed A. Desai Book 2000 Humana Press 2000

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Countercurrent Chromatography of Proteins with Polymer Phase Systems,olvent systems consist of either one polymer component such as polyethylene glycol (PEG) and a high concentration of salt such as potassium phosphate, or two different polymers such as PEG and dextran in water. Being free from the organic solvent, the system can preserve a natural structure of prote

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Sterile Filtration and Virus Filtration,peutics (e.g., monoclonal antibodies, recombinant gene proteins, and plasma components). Membrane filtration is attractive for both bacterial removal and viral clearance because it is simple to operate and causes minimal damage, even for products that are highly labile to heat, radiation, or chemica

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Validation of Sterilizing Filters in the Biotechnology Industry,ization. However, some parenteral drugs were heat sensitive and could not be sterilized by heat. This was the beginning of the search for alternative methods of sterilization. Filtering out contaminating organisms from the drug product was obviously an excellent idea and filters made of a variety of

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Benjamín Vargas-Quesada,Félix de Moya-Anegón that can be recovered by physical separation from nonassociated contaminants. Of course, if a suitably efficient protein refolding strategy is not available (.), then any gains achieved through inclusion body formation may be easily lost.

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New Directions in Latino American Culturesgulated pharmaceutical industry, through the production of industrial specialty enzymes, to the low-cost high-volume applications characteristic for the food industry. Even certain applications within the field of waste water treatment and valorization of waste materials may be envisioned.

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Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation, that can be recovered by physical separation from nonassociated contaminants. Of course, if a suitably efficient protein refolding strategy is not available (.), then any gains achieved through inclusion body formation may be easily lost.

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