Titlebook: Directed Evolution; Methods and Protocol Andrew Currin,Neil Swainston Book 2022 Springer Science+Business Media, LLC, part of Springer Natu

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Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity,tant metabolites, therefore presenting applications in the bioremediation, industrial biotechnology, and biomedical fields. The directed evolution of allosteric transcription factors (aTFs) with the aim of altering effector specificity has the potential for the development of new biosensors to detec

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A Screening Method for P450 BM3 Mutant Libraries Using Multiplexed Capillary Electrophoresis for Deameter capillaries or micro- and nanofluidic channels through the application of a high voltage electric field. When capillary electrophoresis is assembled in a multicapillary instrument such as 96-well format (multiplexed), it becomes a powerful high-throughput system with the ability to simultaneo

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Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screenic and industrial interest. Here we describe an ultra-high-throughput fluorescence activated cell sorting (FACS) method for the directed evolution of GTs, at the single cell level. This assay relies on the exquisite substrate specificity of lactose permeases (LacY) that are located in the cell membra

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