| 书目名称 | Directed Evolution | | 副标题 | Methods and Protocol | | 编辑 | Andrew Currin,Neil Swainston | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Cutting-edge and comprehensive, .Directed Evolution: Methods and Protocols. is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.. | | 出版日期 | Book 2022 | | 关键词 | SelFi; computationally guided protein stapling; De novo protein design; gene synthesis; mutagenesis | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-2152-3 | | isbn_softcover | 978-1-0716-2154-7 | | isbn_ebook | 978-1-0716-2152-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2022 |
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发表于 2025-3-21 19:04:37
