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Titlebook: Diffusion Chamber Culture; Hemopoiesis, Cloning Eugene P. Cronkite,Arland L. Carsten Conference proceedings 1980 Springer-Verlag Berlin Hei

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Gayani Karunasena,Richard Tucker,Susan Angmean number of endoreduplications per colony megakaryocyte and the number of doublings undergone by the colony progenitor. Megakaryocytes can also be grown in diffusion chambers although with a lower efficiency and smaller colony size than in plasma clot cultures.
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https://doi.org/10.1007/978-1-4612-4806-4l development. The results suggest that the CFU-D7 represents an earlier progenitor cell than the CFU-D4 in the differentiation pathway of the granulocyte which in some respects displays behavior similar to that of the CFU-S.
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Marrow Culture in Diffusion Chambers in Rabbits IV. Effects of Leukocyte Products on Granulopoiesis did not stimulate granulopoiesis, but did increase chamber macrophage/mononuclear cell production. Partial purification of LP reveals that granulopoietic stimulation is produced by material having a molecular weight less than 50000 daltons. Intravenous injection of LP material greater than 50000 daltons inhibited chamber granulocyte production.
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Gayani Karunasena,Richard Tucker,Susan Angion. Mature plasma cells do not and immature plasma cells do proliferate. Estimation of magnitude of plasma cells formed in the cultures at day 18 indicated that at least one plasma cell is formed for every six normal human blood lymphocytes introduced into the culture.
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Human Granulocytopoietic Progenitor Cell Diversity and Regulation Studied in Diffusion Chambersulated by species nonspecific diffusible factors in irradiated mice (or inhibited by factors in nonirradiated mice). These studies suggest that an analogy exists between the organization of erythroid and neutrophilic progenitor cells.
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Culture of Normal Human Blood Cells in a Diffusion Chamber System II. Lymphocyte and Plasma Cell Kinion. Mature plasma cells do not and immature plasma cells do proliferate. Estimation of magnitude of plasma cells formed in the cultures at day 18 indicated that at least one plasma cell is formed for every six normal human blood lymphocytes introduced into the culture.
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Conference proceedings 1980 to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitu
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