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Titlebook: Diffusion Chamber Culture; Hemopoiesis, Cloning Eugene P. Cronkite,Arland L. Carsten Conference proceedings 1980 Springer-Verlag Berlin Hei

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书目名称Diffusion Chamber Culture
副标题Hemopoiesis, Cloning
编辑Eugene P. Cronkite,Arland L. Carsten
视频video
图书封面Titlebook: Diffusion Chamber Culture; Hemopoiesis, Cloning Eugene P. Cronkite,Arland L. Carsten Conference proceedings 1980 Springer-Verlag Berlin Hei
描述Even though diffusion chamber culture was commenced long before ortho­ dox tissue culture by Metchnikoff (1887) there have been only sporadic attempts to use this methodology to study cell proliferation (review by Carsten, Chap. 1). Not so long ago diffusion chamber culture was nicknamed "confusion chamber" culture. I believe this conference has removed the confusion and will truly point out the intrinsic value of the system. It is not a substitute for established in vitro culture nor for in vivo studies. It complements both. Dr. Arne B~yum introduced diffusion chamber culture at Brookhaven National Laboratory. After some modest success in showing that one could culture human bone marrow and with appropriate stimuli induce erythro­ poiesis in diffusion chambers, several of the participants at this conference visited Brookhaven to learn firsthand this simple technology and to apply it in their own laboratories. However, the technique did not spread widely and controversy arose in which the same question was repeatedly asked: Can the diffusion chamber technique provide information that is not ob­ tainable more rapidly and easily, and at less expense by the in vitro tech­ niques? As a
出版日期Conference proceedings 1980
关键词Diffusion; cell; cytogenetics; genetics; leukemia; mutagen; tumor
版次1
doihttps://doi.org/10.1007/978-3-642-67644-4
isbn_softcover978-3-540-10064-5
isbn_ebook978-3-642-67644-4
copyrightSpringer-Verlag Berlin Heidelberg 1980
The information of publication is updating

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Roles of CFU-S and CFU-C in Maintaining Cell Growth in Diffusion Chamber Cultures of Murine Marrowsal low levels characteristic of the normal marrow. Experiments were then performed to determine the effect of killing most of the CFU-C while preserving most of the CFU-S in plateau phase cultures by incubating the cultured cells with Ara-C followed by re-culture in new DCs and new host mice. In co
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Studies on the Regulation of Diffusion Chamber Granulopoiesisurther studies evaluated the DC growth of murine marrow depleted of CFU-S by in vitro exposure to absorbed mouse brain antiserum plus complement. There was little effect of this treatment on DC-differentiated or stem cell recovery. These results raise questions with regard to the nature of the DC st
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Growth of Canine Bone Marrow and Peripheral Blood Mononuclear Cells in In Vivo Plasma Clot Diffusion macrophages. The cellular growth curves of BM in LDC and PCDC cultures were markedly different. Total nucleated cells per DC in liquid cultures decreased after implantation to a minimum value at day 4 and then rose to control levels by days 7–8. In contrast, the cellularity of PCDC cultures increas
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Stimulating Factor(s) of Hematopoiesis in the Bone Marrow of Mice Administered Hydroxyurea. Effects ence of a stimulating factor(s) of granulopoiesis was manifested by (1) a higher granulocyte/macrophage (G/M) ratio in single DC containing HU-treated bone marrow than in control cultures and (2) a higher G/M ratio in both compartments of the DDC in experimental groups than in controls. The stimulat
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Use of Diffusion Chambers for Studying Cell Interactions: Sequential Studies of Eosinophil Granulocyties, total CBM noneosinophil granulocytes (NEG), and CBM percent NEG did not differ as a function of the origin of the respective transfilter spleen cells over a 9-day period. Values for total eosinophils, percent eosinophils, and the percentage of granulocytes that were eosinophils were significan
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