Titlebook: Diabetic Nephropathy; Methods and Protocol Luigi Gnudi,David A. Long Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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Detlef Pollack,Volkhard Krech,Markus Heromergence of numerous tissue clearing strategies, and advancements in confocal and two-photon microscopy, enabling the study of intact organs and systems down to cellular and sub-cellular resolution. In this chapter, we describe a wholemount immunofluorescence technique for labeling structures in ren

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Maike Rönnau-Böse,Klaus Fröhlich-Gildhoffn in general and also to specific capillary beds including the renal glomerular capillaries. Endothelial dysfunction, including increased permeability, is a key component of diabetes-induced organ damage. Endothelial cells together with their glycocalyx, grown on porous membranes, provide an excelle

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https://doi.org/10.1007/978-3-531-92775-6lumen. The latter is sensed by intrinsic ER transmembrane proteins: IRE1α (inositol-requiring protein-1α), PERK (protein kinase RNA (PKR)-like ER kinase), and ATF6 (activating transcription factor 6) which when activated trigger the unfolded protein response (UPR), which includes an inhibition of pr

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https://doi.org/10.1007/978-3-658-16253-5eflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease..Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. T

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